Abstract: Aiming at the problems of soil pollution and increase of nematodes caused by continuous cropping in facility agriculture, a rotary tillage soil disinfection machine based on ozone technology was designed. The structural parameters of the rotary tillage soil disinfection machine was designed and calculated, the force of the ozone inflatable shovel structure under the action of motion was analyzed, the kinematic conditions for ozone injection into the soil was obtained and the structural parameters of the inflatable shovel was determined. The XC2-24R-C controller was used as the core component of the circuit control module, and the ozone was extracted by high voltage discharge method to realize the normal operation of the ozone generator. The SolidWorks software was used to build the whole machine structure model, and the prototype was developed and tested. Using the root-knot nematodes as a test, under the same experimental conditions, the nematode regression rate of conventional tillage was 2.96%, and the maximum nematode regression rate and control efficiency of root-knot nematode were 84.93% and 84.47% after ozone disinfection, which was 81.97% higher than that of conventional tillage.