Abstract: In order to obtain the accurate discrete element parameters of tobacco nutrient soil, the simulation parameter calibration of tobacco nutrient soil discrete element was carried out by combining simulation test and bench test. Taking the Angle of repose of tobacco vegetative soil as the response index and the Hertz-Mindli（no slip） discrete element contact model was used to design the Plackett-Burman test, to screen the discrete element simulation parameters of tobacco vegetative soil. The factors that had significant influence on the resting Angle of tobacco vegetative soil were the static friction coefficient between vegetative soil, the rolling friction coefficient between vegetative soil and the static friction coefficient between vegetative soil and glass. In order to further obtain the optimal parameter combination of each factor, Box-Behnken experiment with three factors and three levels was designed, and the second-order regression response model of the rest-angle parameters of tobacco vegetative soil was obtained. The results showed that the optimal parameter combination of discrete element model of tobacco nutrient soil was as follows: static friction coefficient of 0.67, rolling friction coefficient of 0.35, static friction coefficient of vegetative soil and glass of 0.35. Under the discrete element model obtained from calibration, the relative error of the resting Angle obtained by simulation test and bench test is 2.59%.