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磁化咸水灌溉对葡萄幼苗生长及离子稳态的影响

Effect of irrigation with magnetized saline water on grape seedlings and ionic homeostasis

  • 摘要: 以‘夏黑’葡萄扦插苗为试材,采用不同矿化度咸水(0、3.0、6.0 g/L)经磁化和非磁化处理后用于灌溉葡萄幼苗,以探讨磁化咸水灌溉对葡萄幼苗生长和不同种类阳离子分布特征的影响。采用收获法和原子吸收分光光度法对葡萄生物学特性和不同组织Na+、K+、Ca2+、Mg2+质量分数进行了测定。研究结果表明:1)与对照(0,NM0)相比,非磁化咸水灌溉均抑制了葡萄的生长和生物量积累,其中,6.0 g/L咸水灌溉下葡萄存活率降低。磁化咸水灌溉(0、3.0和6.0 g/L)则提高葡萄的新梢生长量、生物量、根冠比和存活率,降低葡萄各器官的盐敏感指数。2)咸水灌溉下葡萄体内Na+质量分数显著提高(P < 0.05),K+/Na+、Ca2+/Na+、Mg2+/Na+显著降低(P < 0.05);与非磁化咸水灌溉相比,磁化咸水灌溉降低了葡萄体内Na+质量分数,提高了葡萄茎和根系的拒Na+能力以及对K+、Ca2+、Mg2+的吸收和运输能力,同时,促进了Ca2+、Mg2+在叶片中的累积。因此,磁化处理不仅可以降低盐分胁迫对葡萄的生长抑制,而且促进植物体内离子稳态的重建,且为咸水资源的开发利用及磁化水处理技术的推广应用提供了理论依据。

     

    Abstract:
    Background Plant growth can be inhibited by salinity conditions, which could lead to an osmotic stress and ion poisoning, hence causing decreases in yield and quality of crops. The magnetic technology, as a primer eco-friendly technique, can be used to the exploration and utilization of saline water. Consequently, the magnetic saline water could contribute to reduce the increasing pressure on freshwater resources in irrigation farming, especially in saline areas.
    Methods A pot experiment was carried out in a greenhouse, and two-year-old seedlings of Vitis vinifera×V. labrusca 'Summer Black' were used as the test materials. The irrigational experiments were performed by different concentration of NaCl solution (0, 3.0 and 6.0 g/L) under magnetic and non-magnetic treatments. The characteristics of biomass, survival ratio, salt sensitive index, roots morphology parameters and ionic content (Na+, K+, Ca2+ and Mg2+) were determined using harvested method and atomic absorption spectrometry (AAS). The aim in our study is to explore the effect of saline water on grape seedling growth under magnetic field.
    Results 1) The seedling growth and biomass of grapes were suppressed after suffering to saline conditions, while the survival ratio of the seedlings decreased in the treatment of 6.0 g/L NaCl solution. However, the irrigation with magnetized water stimulated the seedling growth, increased the shoots length, biomass, root/shoot ratio and survival ratio, while decreased the salt sensitive index. 2) The Na+ contents in grape seedlings raised with the increase of NaCl solution (P < 0.05), whereas the ratios of K+/Na+, Ca2+/Na+, Mg2+/Na+ significantly decreased (P < 0.05). Differing from increasing content of Na+ in non-magnetized water managements, magnetically treated water resulted in a lower Na+ contents, but an enhancement in the ability of rejecting Na+ in grape stem and root as well as in the absorption and transportation of K+, Ca2+ and Mg2+, also increases in the accumulation of Ca2+ and Mg2+ in leaves.
    Conclusions From the above results, we could see that, the seedling growth is improved when irrigating with magnetized saline water, also the salt tolerance and ion homeostasis of grapevines is enhanced by impacting of magnetic treatment. And these positive influences induced by the magnetic field would provide a theoretical foundation for the application of the magnetic technology in saline areas and saline water management.

     

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