Abstract: The plant expression vector of pDREB-PMI was constructed successfully with expression vector of prd29a-dreb-hyg and pZMLR14 by restriction-ligase reaction. Sugarcane callus were transformed with pDREB-PMI expression vector by bombardment. After mannose selection, 51 regenerated plants were obtained with the transformation rate of 4.25%. All of these 51 seedlings were detected with molecular detection, and the 8 seedlings were positive transgenic sugarcane. Chlorophenol red assay of transgenic sugarcane leaf showed that the pmi gene was expressed in transgenic plants. T₁-generation transgenic sugarcane were detected by PCR and RT-PCR, and the exogenous gene EaDREB2B was stably inherited. The results laid the foundation of further research on the effect of EaDREB2B gene in improving sugarcane resistance to drought stress.