牡丹胚培养快速成苗技术研究

徐莉; 成仿云; 钟原

doi:10.7525/j.issn.1673-5102.2017.05.008

牡丹胚培养快速成苗技术研究

Study on Rapid Seedling-raising Technology of Tree Peony Embryo Culture

摘要

摘要: 以‘凤丹’及紫斑牡丹种胚为外植体，通过研究激素、光照条件、培养基成分及活性炭（AC）等对离体胚培养的影响，建立了牡丹胚培养直接成苗的高效、快捷的方法。主要结果：在改良MS（钙加倍）+0.2 g·L^-1AC+0.5 mg·L^-1BA+0.5 mg·L^-1GA中离体胚10 d胚根萌动，20 d长出真叶，25 d根系发达，40 d移栽，3个月后幼苗长势良好。实验得出适宜‘凤丹’的培养基：改良MS（钙加倍、大量元素加倍）+0.6 g·L^-1AC+1.0 mg·L^-1GA，紫斑牡丹：改良MS（钙加倍、大量元素加倍）+0.6 g·L^-1AC+0.5 mg·L^-1GA；其中‘凤丹’的成苗率63.88%，成活率66.34%，紫斑成苗率93.75%，成活率在品种间有差异，最佳可达100%。

Abstract: This study used Paeonia ostii ‘Fengdan’ and Paeonia rockii as explants, established efficient method of Seedling-raising of tree peony embryo culture through the study of hormones, light conditions, medium ingredients and activated carbon(AC) on the in vitro embryo culture. The main result:In vitro embryos radicle germination in 10 d, grow true leaves in 20 d, root system developed in 25 d, transplanting in 40 d, after 3 months the seedlings grow well in the medium of improved MS(calcium doubled)+0.2 g·L^-1AC+0.5 mg·L^-1GA+0.5 mg·L^-1BA. Suitable medium for ‘Fengdan’:improved MS(calcium doubled,macroelement doubled)+0.6 g·L^-1AC+1.0 mg·L^-1GA; medium for P.rockii:improved MS(calcium doubled,macroelement doubled)+0.6 g·L^-1 AC+0.5 mg·L^-1GA; The seedling rate of ‘Fengdan’ was 63.88%, the survival rate was 66.34%, and the rate of seedling of P.rockii was 93.75%, and survival rates vary among breeds, with a maximum of 100%.

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