Abstract: In this work, ultra turrax-microwave assisted extraction process was conducted to extract oil from Pinus koraiensis using ethanol as the extraction solvent. The effects of homogenization speed, homogenization time, liquid-solid ratio, microwave temperature, microwave power, and microwave time on the oil yield were investigated. The optimal conditions for the oil extraction from P.koraiensis seed were obtained as follows:homogeneous speed 12 000 r·min^-1, homogeneous time 120 s, the ratio of liquid to material 20 mL·g^-1, microwave temperature 60℃, microwave power 700 W and microwave time 50 min. Under the above conditions, the optimum oil yield was 60.3%. The fatty acid composition of P.koraiensis seed oil was analyzed by GC-MS, and the main components were identified as linoleic acid, stearic acid and palmitic acid. The contents of unsaturated fatty acid and specific pinolenic acid reached 85.55% and 13.65%, respectively. The antioxidant activity and physicochemical properties of P.koraiensis seed oil were also determined. The seed oil exhibited better DPPH radical scavenging activity with the IC₅₀ value of 0.0954 g·mL^-1. And the seed oil was a high quality natural oil with lower acid value, lower peroxide value and high iodine value.