Abstract:
According to the transcriptome data of
Bambusa emeiensis shoot, two
bZIP genes designated as
BebZIP2 and
BebZIP6 were cloned and their bioinformatics were analyzed. The bioinformatics analysis results showed that the full-length cDNA sequence of
BebZIP2 and
BebZIP6 were 504 and 720 bp, and encoded 167 and 239 amino acids, respectively. BebZIPs and rice OsbZIP52/RISB5 proteins were clustered into the same branch, which were bZIP-related proteins. The tissue expression patterns of two
BebZIP genes were analyzed by real-time PCR. Two
BebZIP gene sequences were expressed in all shoots, stalks, unfolding leaves and rolled leaves of
B.emeiensis. There were the expression differences of the same gene in the different tissues and expression quantity in the descending order was unfolding leaves, rolled leaves, stalks and shoots. The seedlings of
B.emeiensis were treated with ABA, NaCl and PEG 6000 for abiotic stress analysis. The expression level of
BebZIP2 and
BebZIP6 genes treated by salt, drought and ABA stresses have different degree of sensitivity.