Abstract: One-year-old twigs of 58 Larix olgensis clones were taken as materials and low temperature stress experiment were conducted to discuss the cold resistance ability of different L.olgensis clones. Different relative conductivity were found in four L.olgensis clones under different time and temperature treatment, the trance were increased with the temperature decreased and stress time extend. With the same stress(-40℃, 12 h) condition, the relative conductivity showed significant different among 58 L.olgensis clones(P<0.01). With the difference of relative conductivity between treatment(-40℃, 12 h) and control as analysis trait, 9 strong cold resistance clones were selected(L5, L16, L21, L23, L27, L40, L73, L78 and L90) by hierarchical clustering. These clones could be considered as excellent cold resistance clones and the research can supply basis for improved variety breeding of cold stress in L.olgensis.