Abstract: The nucleotide sequences of FmS6K(FmS6K1 and FmS6K2) gene and the basic information of protein encoded by FmS6K gene were analyzed, and the characteristic of gene expression under abiotic stresses and plant hormones were studied. The length of FmS6K1 and FmS6K2 genes were 1 583 and 1 796 bp, encoding 480 and 483 amino acids, respectively, both of which contained a complete open reading frame. FmS6K1 is an unstable amphipathic protein, meanwhile FmS6K2 is a labile hydrophilic protein, neither of which has a signal peptide. The expression levels of FmS6K1 and FmS6K2 genes were affected by both low temperature(4℃) and salt(NaCl) stress. Compared to low temperature, FmS6K1 and FmS6K2 genes in roots, stems and leaves were more sensitive to salt stress. In FmS6K1 and FmS6K2 genes, the later was more sensitive to low temperature and salt stress. Exogenous ABA, GA and IAA could affect the expression of FmS6K1 and FmS6K2 genes. The expression level of FmS6K1 gene in roots, stems and leaves was higher in IAA treatment comparing to ABA and GA treatment. The response of FmS6K2 to exogenous GA and ABA was more obvious in root, while that to exogenous IAA and ABA was more significant in stem and leaf. The above results indicated that the FmS6K gene played an important role in regulating plant growth and development and adapting to adversity stresses.