Abstract: Characteristics of leaf epidermis of Meconopsis integrifolia were observed under optical microscope, including trichome, stomata and epidermis cell. The relationship between epidermis trichome, stomata, epidermis cell of leaf and altitude was discussed. The trichome density, stomata density and epidermis cell density of both upper and lower leaf epidermis increased gradually as the altitude increased. While the length, width and area of both stomata and epidermis cell decreased. Morphology of epidermis cell changed from irregular to polygonal. The shape of anticlinal wall altered from corrugated to arched or straight. These variation of characteristics probably be a general response of plants which living under plateau environment in a long-term, providing theoretical support for further study on alpine plant adaptation.