化学诱变剂EMS对知母种子萌发的影响

Effects of EMS on Seed Germination of Anemarrhena asphodeloides in the Simulated Environment

摘要: 以知母（Anemarrhena asphodeloides）种子为材料，分别采用1%、2%、4%、6%浓度的EMS处理6、8、12和24 h，在恒温培养箱内进行种子萌发实验，研究不同浓度的化学诱变剂甲基磺酸乙酯（ethyl methane sulfonate，EMS）处理对知母种子萌发的影响，筛选适宜的诱变浓度和诱变时间。结果表明：随EMS浓度的增加和处理时间的延长，知母种子发芽率呈现逐渐降低的趋势，以相对发芽率达到半致死浓度为标准，6%EMS浸种12和24 h可作为EMS诱导知母建立突变体库的适宜条件。

Abstract: The seeds of Anemarrhena asphodeloides were treated with different contents and treatment times of EMS at 1%, 2%, 4%, 6% and 6, 8, 12, 24 h, respectively, in the constant temperature incubator in this experiment.We studied the effects of the different concentrations and treatment time of EMS on the seed germination characteristics of A.asphodeloides. The results of seed germination showed that the seed germination of A.asphodeloides reduced with theincreasings of the contents and the treatment time of EMS. With the relative germination rate reaching the semi-lethal concentration as the standard, the suitable condition for EMS treatment of A.asphodeloides seeds are the concentration of 6% and soaking seeds for 12 and 24 hours.