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湿加松无性系表型遗传多样性研究

Phenotypic Genetic Diversity of Pinus elliottii×P.caribaea Morelet var. hondurensis Clones

  • 摘要: 以35个湿加松(Pinus elliottii×P.caribaea Morelet var.hondurensis)无性系为试验材料,对其8个表型性状进行相关性分析、聚类分析及主成分分析研究,并深入探讨了遗传多样性水平,为湿加松无性系利用及良种选育提供科学依据。结果表明:(1)生长性状变异系数介于12.31~29.28%,均值为22.90%;多样性指数介于1.61~1.80,均值为1.69。形质性状变异系数介于13.34~47.25%,均值为26.86%;多样性指数介于1.50~1.94,均值为1.77。表明湿加松无性系遗传变异较为丰富,遗传多样性水平较高。(2)8对表型性状两两间有11对呈极显著正相关,5对呈显著正相关。选定材积为该材料育种选择的主要指标,其次为树高。(3)采用系统聚类法在欧式距离为12的时候将35个无性系分为6大类群,部分来源相同或父本相同的聚为一类,说明它们在表型上的变异较小。(4)针对8个表型性状做主成分分析提取了3个主成分,累积贡献率达86.00%,主要代表了6个性状,以此来表达所有供试材料的综合变异情况。

     

    Abstract: Thirty-five Pinus elliottii×P.caribaea Morelet var. hondurensis clones were used as experimental materials, and correlation analysis, cluster analysis and principal component analysis were performed on eight phenotypic traits to explore genetic diversity level. The level of genetic diversity was discussed to provide scientific basis for the utilization of P.elliottii×P.caribaea Morelet var. hondurensis clones and breeding of improved varieties. The results showed that: (1)The coefficient of variation of growth traits ranged from 12.31%-29.28%, the mean value was 22.90%, and the diversity index ranged from 1.61-1.80 with an average of 1.69. The coefficient of variation of shape traits was between 13.34% and 47.25%, the mean value was 26.86, and the diversity index was between 1.50 and 1.94, with a mean value of 1.77. The P.elliottii×P.caribaea Morelet var. hondurensis clones was rich in genetic variation and high in genetic diversity. (2)Among the eight pairs of phenotypic traits, eleven pairs of trait correlations were extremely positively significant, and five pairs of them were positively significant. The selected volume was the main index for breeding selection of the material, followed by tree height. (3)When the Euclidean distance was 12, thirty-five clones were divided into six groups, and some of them come from the same source or the same male parent. (4)Three principal components were extracted from the principal component analysis of eight phenotypic traits, and the cumulative contribution rate reached 86.00%, which mainly represented six traits, so as to express the comprehensive variation of all the tested materials.

     

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