Abstract: LOX(lipoxygenase) is a kind of oxygenase widely distributed in plants and animals, which are involved extensively in plant growth, development and abiotic and biotic stress. A genomed-wide search was carried out to identify LOX genes in Eucommia ulmoides, we also analyzed the LOX genes structure, localization, and chemicophysical properties, classification and function of protein by bioinformatics method. The E.ulmoides genome contained 23 putative LOX genes clustered into two groups, designated 9-LOX and 13-LOX, but only one LOX belonged to 9-LOX, and other 22 LOX belonged to 13-LOX, were targeted to cytoplasm or chloroplast. These LOX genes were located on the 12 scaffolds in non-random ways, mostly grouped together within single scaffold. RNA-Seq showed 6 13-LOX genes were not expressed in bark, fruit, leaf and kernel, while the expression of other 17 LOX genes were different in four organs from different developmental stages. The results of this investigation could definitely provide a significant foundation for further research on function analysis of E.ulmoides LOX gene family.