Abstract: In order to explore the differences in the metabolites and metabolic pathways of leaves from wild Rosa acicularis and R. acicularis‘Luhe’,a GC-MS non-targeted metabolomics test was carried out on the leaves of twokinds of R. acicularis,6 amino acids,12 sugars,four alkanes,17 organic acids,five esters and seven othercompounds and a total of 51 metabolites were detected respectively. The PCA model was in line withexpectations and the OPLS-DA model screened out 19 significantly different metabolites. The analysis ofdifferential metabolic pathways revealed a total of five major differential metabolic pathways. Based on theresults,there were significant differences in the metabolism of some organic acids and sugars in as well as in theABC transporter,phenylpropane biosynthesis,benzoic acid degradation and the metabolism of alanine,aspartic acid and glutamic acid in the leaves of the two species of R. acicularis. It was speculated that there weresignificant differences in leaf photosynthetic efficiency and resistance to stress,but further study was needed incombination with genome,proteome,transcriptome and other methods.