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不同激素处理对白桦幼树萌条及三萜合成的影响

Effects of Different Hormone Treatments on Sprouting Shoots and Triterpenoids Synthesis of Betula platyphylla Suk.

  • 摘要: 探索一种可持续、稳定的获得白桦(Betula platyphylla Suk.)枝条的方法,拟通过促萌处理建立多枝条类型白桦材料。以一年生白桦幼苗为试材,采用3个截干高度:10、20、30 cm处理后,每个截干高度使用4种激素或化学药剂处理(20 mg·L-12,4-D、20 mg·L-1KT、20 mg·L-16-BA和20 mg·L-1KMnO4)进行促发萌条试验。结果表明,白桦幼苗初次截干(30 d后)采条,截干高度10 cm进行激素KT、6-BA处理的白桦苗萌芽率最高,达65.0%以上;锯末保湿处理下3种截干高度萌条率、萌条产量均较高,萌条率达75.0%以上。枝条中总三萜含量以截干高度20 cm处理下最高,达76.43 mg·g-1,其次为截干高度10 cm处理下,其他处理均不利于总三萜积累。叶中总三萜含量以截干高度30 cm下激素6-BA处理最高,达91.16 mg·g-1,是锯末处理(30 cm)的2.68倍。在锯末处理、KT和KMnO4处理下的枝条和叶片中总三萜含量显著低于其他处理。6-BA对枝条、叶片中齐墩果酸的积累有利,其他处理均不利于齐墩果酸积累。锯末处理有利于二次萌条及产量的提高,截干高度为10 cm处理下萌条产量达126.96 g。枝条总三萜含量以截干高度20 cm进行激素6-BA处理效果较好,达97.99 mg·g-1。叶中总三萜含量以截干高度30 cm进行激素KT处理最佳,达99.22 mg·g-1,其次为锯末处理组和6-BA处理组。该研究结果为可持续、高效的利用白桦资源及获得三萜产物提供了技术支持。

     

    Abstract: To explore a sustainable and stable way to obtain birch branches,a multi branch birch by promoting germination was established. One year old birch seedlings were used as experimental materials,and three cutting heights(10,20 and 30 cm)and four hormone or chemical agents(20 mg·L-12,4-D,20 mg·L-1 KT,20 mg·L-16-BA and 20 mg·L-1 KMnO4)were used to promote the sprouting of birch seedlings. The results showed that the germination rate of birch seedlings was the highest,which reached 65.0% after the first cutting(30 d)and the cutting height was 10 cm. Under the treatment of sawdust moisture,the sprouting rate and sprouting yield of the three cutting heights were higher,the sprouting rate was more than 75.0%,and the yield was more than 77.0 g per plant. The content of total triterpenoids in the branches was the highest at 20 cm cutting height(76.43 mg·g-1),followed by 10 cm cutting height. Other treatments were not conducive to the accumulation of total triterpenoids. The content of total triterpenoids in leaves was the highest under the treatment of 6-BA(91.16 mg·g-1),which was more than 2.68 times of sawdust treatment(30 cm). The content of total triterpenoids in the branches and leaves of sawdust,KT and KMnO4 treatments was significantly lower than that of other treatments. 6-BA was beneficial to the accumulation of oleanolic acid in branches and leaves,while other treatments were not conducive to the accumulation of oleanolic acid. Sawdust treatment was beneficial to the secondary sprouting and yield improvement. The sprouting yield of the treatment with 10 cm cutting height reached 126.96 g per plant. The total triterpenoids content in the branches was 97.99 mg·g-1 when treated with6-BA at the cutting height of 30 cm. The content of total triterpenoids in leaves was option when the cutting height was 20 cm,reaching 99.22 mg·g-1,followed by sawdust treatment group. The results provid technical support for sustainable and efficient utilization of birch resources and obtaining triterpenoids.

     

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