Abstract: To reveal the variation of leaf phenotypic diversity of Tetrastigma hemsleyanum Diels＆Gilg from different origin and its correlation with the total flavonoids content of overground part.In this paper,49germplasm resources（abbreviation resources）of T.hemsleyanum were selected as materials,and 12 characters of length and 7 proportion characters of leaf phenotype were compared.The phenotypic characters of leaf and their relationship with origin location and total flavonoids content were statistically analyzed by correlation analysis,factor analysis and cluster analysis.The results showed that there were significant differences in leaf length characters among resources,and the leaf characters of petiolule and lateral leaflet were of high reference value in the identification of T.hemsleyanum resources;There was correlation between leaf length characters and proportion characters,which showed that leaflet was wider when petiole grew,and leaflet was longer and narrower while leaflet elongated;Some leaf shape characters,such as central leaflet length and lateral petiolule length,were significantly（P＜0.05）or extremely significantly（P＜0.01）correlated with latitude,longitude or altitude of origin location,which indicated that these characters could be used as preliminary basis for geographical location estimation of origin;The cluster analysis based on 3 common factors of leaf shape showed that the leaf shape of 49 resources of T.hemsleyanum could be divided into five types,and the number of leaf type in each origin（municipalities）showed a radially decreasing trend from Jiangxi to the surrounding area;There was no significant correlation between the total flavonoids content of the overground parts and the geographical location of origin,but total flavonoids content was significantly negatively correlated（P＜0.01）with the common factor of leaf blade characters（common factor 1）,and significantly positively correlated（P＜0.01）with the common factor of petiole length characters（common factor 2）.The results improved the morphological data of T.hemsleyanum and provided the morphological basis for the breeding of excellent varieties of T.hemsleyanum.