Abstract: The cuticle is an impermeable fatty layer on the surface of the epidermal cell wall,and closelycombined with epidermal cells,the micromorphological characteristics such as the morphology and arrangementof plant epidermal cells,as well as the morphological structure of the stomatal apparatus,can be reflected inthe cuticle.In this paper,the cuticular micromorphology of needles of 12 species of Pinus was observed andcompared by optical microscope and scanning electron microscope,and 20 characters were described in detail,of which 12 characters came from the inner surface of the cuticles and eight characters from the outer surface ofthe cuticles respectively.The results showed that these characters had taxonomic importance for the intra-genusclassification and the identification of similar species:（1）Micromorphological features of the cuticle such as thelength of epidermal cells,the length of bristle,the undulating degree of the outer surface of the cuticle,theoutline of epidermal cells,the presence or absence of stomatal plug and needle-like substances had theirspecificities,which could be used as a basis for the classification of Pinus at the section level.The micromorphological features of cuticles did not support the view of merging P.Section Cembra and P.SectionParrya into P.Section Quinquefolius,nor did it support the view of dividing P.Section Pinus into P.SectionPinus and P.Section Trifolius.（2）The cuticle micromorphological characteristics of the needles of P.bungeana were partly the same as P.Section Cembra,partly similar to P.Section Pinus,and partly different from otherspecies of Pinus,which could provide a new argument for the establishment of P.Subgenus Parrya.（3）Underscanning electron microscope,the anticlinal wall pattern of epidermal cells,the existence or nonexistence ofstomatal plug and the stomatal shape of the outer surface could provide interspecific delimitation basis formorphologically similar species of P.taeda and P.elliottii.