Abstract: In order to explore the obstacle factors of regeneration of Amygdalus ledebouriana in Xinjiang, and to better protect the germplasm resources and population, the seeds of A. ledebouriana in Barluk Mountain of Xinjiang were used as experimental materials, and the effects of seed water absorption characteristics, different stacking time, different concentrations of GA3 and different substrate treatments on the dormancy and germination characteristics of A. ledebouriana seeds and the germination inhibitors in A. ledebouriana were explored respectively. The results showed that:（1）The natural dormancy of whole seeds was relieved after 80 days stratification, while the decorticated seeds could germinate without stratification, and the addition of 300 mg·L^-1 G_A3 could break the dormancy of decorticated seeds in advance and improve the germination rate.（2）The germination inhibitors in A. ledebouriana seeds mainly existed in exocarp and mesocarp.（3）The germination rate of A. ledebouriana seeds in the original habitat soil was higher than that in the nutrient soil.