Abstract: Handeliodendron bodinieri is an endangered species endemic in karst areas of southwest China.In order to explore the influence of soil gravel content on the growth and physiological characteristics of H.bodinieri seedlings in three consecutives growing seasons,annual H.bodinieri seedlings were used as experimental materials,different soil gravel content（0（control）,20%,40%,60%,80%） were set,and the growth,photosynthesis and physiological indicators of each group of seedlings were determined and evaluated in three consecutive growth seasons.The results showed that in the first growing season,when the soil gravel content was 60%,the net photosynthetic rate of the seedlings was significantly higher than that of the control group;When the soil gravel content was 40%,the growth rate of seedling diameter,leaf biomass,transpiration rate,stomatal conductance,soluble protein mass fraction,and superoxide dismutase（SOD） activity were significantly higher than those of the control group;The intercellular CO₂ molar fraction and soluble sugar mass fraction of seedlings were significantly higher than those of the control group when the gravel content was greater than 20%;There was no significant difference in plant height growth rate,leaf area,root,stem,and total biomass between the control group and the control group.In the second growing season,when the soil gravel content was 60%,the transpiration rate and soluble protein mass fraction of H.bodinieri seedlings were significantly higher than those of the control group;When the soil gravel content was 40%,the SOD activity of the seedlings was significantly higher than the control group,and the stem biomass was significantly higher than the control group when the soil gravel content was 20%.The other indicators showed similar trends to the first growth season.With the extension of the growing season,in the third growing season,the growth rate of plant height and ground diameter,stem biomass,leaf area,stomatal conductance,SOD and POD activities of H.bodinieri seedlings reached the maximum when the soil gravel content was 60%,which was significantly higher than that of the control group;The net photosynthetic rate and intercellular CO₂ concentration of seedlings reached the maximum when the soil gravel content was 40%,which was significantly higher than that of the control group.The comprehensive analysis showed that in the three consecutive growth seasons,the soil gravel content with the highest score of H.bodinieri seedlings was 40%,40% and 60% respectively.For three consecutive growth seasons,seedlings grew best at 60% soil gravel content.