Abstract: To explore the effect of BpGLK inhibition on plant chlorophyll content on plant growth, the variation of leaf color of transgenic Betula pendula ’Dalecarlica’ during the growth period was investigated. Two-year-old BpGLK transformed B. pendula ’Dalecarlica’ was used as materials, and the stability and relative expression of exogenous genes, the leaf color parameters, relative chlorophyll content and chlorophyll content were measured respectively, and the temporal variation rule and growth characteristics of leaf color related parameters were analyzed. The results showed that the exogenous gene still existed in the genome of the two-year-old transgenic lines, and the expression level of GLK was significantly down-regulated, during the whole growth period, the leaf color brightness and yellow degree of RE1-RE5 lines were significantly higher than those of WT lines（P＜0.05）, SPAD-value, and the contents of chlorophyll a, chlorophyll b and total chlorophyll were lower or significantly lower than those of WT lines（P＜0.05）, but the ratio of chlorophyll a to chlorophyll b was higher or significantly higher than that of WT lines（P＜0.05）. The plant height of transgenic lines was not significantly affected, and the RE4 and RE5 lines were superior to the other lines in the above traits and were the preferred lines for subsequent landscaping applications.