Abstract: In order to analyze the role of Heat Shock Protein 90（HSP90）in stress and phytohormone signaling transduction in rubber tree,the full-length cDNA sequence of the HbHSP90.4 gene was cloned from rubber tree Reyan73397 latex by PCR,and HbHSP90.4 gene contained a 2 451 bp open reading frame（ORF）and encoded816 amino acids. The results of bioinformatics analysis showed that HbHSP90.4 contained the HSP90superfamily and HATPase superfamily domains,which was a member of the HSP90 family. Phylogenetic analysis revealed that this protein was closely related to cassava MeHSP90. Subcellular localization predictions showed that the HbHSP90.4 gene was localized in endoplasmic reticulum. The qRT-PCR results showed that the HbHSP90.4 gene was mainly expressed in rubber latex. Drought,cold,powdery mildew infection,H₂O₂ and MeJA treatments promoted the up-regulation of the HbHSP90.4 gene in latex respectively,while its expression was significantly down-regulated in ETH,SA and ABA treatments respectively. The plant expression vector HbHSP90.4-mScarlet was also constructed to prepare the material for further transgenic plants. This study laid a solid foundation for the elucidation of the molecular regulation of the latex HbHSP90.4 gene in response to rubber tree stress and the phytohormone signaling pathway.