Abstract: Low temperature is a kind of abiotic stress in the growth process of Hemerocallis fulva.Thetranscriptome data of Hemerocallis fulva leaves under cold treatment groups（10,5,0 ℃）and control group（15℃）was compared,and a total of 2 457 DEGs were screened,including 1 253 up-regulated genes and 1 204 down-regulated genes.The results showed that differentially expressed genes（DEGs）were mainly concentratedin 49 GO processes such as cellular process,metabolic process and catalytic activity,and 42 KEGG metabolicpathways,including metabolic pathways,biosynthesis of secondary metabolites and plant hormone signaltransduction.Among them,the DEGs involved in plant hormone signal transduction pathway changed in varyingdegrees,GH3.10 gene was increased to 13.624 times higher than the control group,IAA1 gene was downregulated to 0.120 times;the DEGs involved in soluble sugar synthesis pathway also changed by 0.076-28.114 times.Then,29 DEGs shared by treatment groups were analyzed by heat map and gene regulation networks.Based on the position of genes in gene regulation networks,the roles of ABCF5,OFPs and SWEETs in responseto cold were analyzed respectively.The results of this study provide a theoretical basis for further exploring thekey genes of low temperature response,germplasm development and molecular breeding of Hemerocallis fulva.