Abstract: The chlorophyll content of the PaGLK gene Populus alba×P.berolinensis inhibited expression line was significantly reduced,and the leaves showed yellow（named golden leaf silver poplar）.Using PaGLK transgenic Populus alba×P.berolinensis,its leaf color parameters and chlorophyll content were measured in time series,and its growth characteristics were analyzed.The results showed that PaGLK transgenic Populus albaX P.berolinensis changed the leaf color,and the chlorophyll content of the expressing lines was significantly lower than that of WT（P＜0.05）,and the leaf brightness was significantly higher than that of WT（P＜0.05）.During the growth and development period,the leaves showed dark yellow green.The daily growth height（GD） of seedling height in the Y2 fast-growing stage of the expressing lines was higher than that of the control lines,and the plant height at the seedling stage was not affected.The over-expressing transgenic Populus alba×P.berolinensis plants were significantly lower than the control plants in that year（P＜0.05）,and the average daily growth height（GD） of the seedling height in the fast-growing period was also 22.19% of that of the control plants.PaGLK inhibited expression strains have potential application value in urban landscaping.