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白桦BpERF98基因的遗传转化及非生物胁迫应答反应

Genetic Transformation of BpERF98 Gene and Abiotic Stress Response of Transgenic Plant in Betula platyphylla

  • 摘要: 非生物胁迫严重影响植物的生长发育,植物通过内部的分子调控机制抵御这种伤害,其中转录因子发挥了至关重要的作用。从野生型白桦(Betula platyphylla)叶片克隆BpERF98基因,通过农杆菌介导法获得过表达BpERF98的转基因白桦植株。测量转基因白桦和野生型白桦在低温、冻害和盐胁迫下的生理指标并进行差异性分析。通过分析发现,在非生物胁迫下转基因白桦的丙二醛含量及相对电导率均低于野生型株系,且转基因株系SOD和POD活性的增长明显高于野生型株系。结果表明,过表达BpERF98基因可以提高白桦对非生物胁迫的抵御能力,这为研究白桦在非生物胁迫下形成的分子机制及白桦抗性分子育种提供了依据。

     

    Abstract: Abiotic stresses seriously affected plant growth and development,and plants had evolved sophisticated internal molecular regulatory mechanisms to deal with,in which transcription factors played a vital role. In this study,BpERF98 gene was cloned from the leaves of Betula platyphylla and overexpressed using Agrobacterium-mediated. The physiological indicators of the transgenic lines and wild-type under low temperature,freezing damage and salt stress were measured respectively,the results showed that the malondialdehyde content and relative conductivity of the transgenic lines under abiotic stress were lower,while the SOD and POD enzyme activities were higher than those of the wild-type. The results showed that overexpression of BpERF98 gene could improve the tolerance of white birch to abiotic stress,and provided an important theoretical basis for studying the molecular mechanism under abiotic stress and molecular breeding for tolerance of white birch.

     

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