Abstract: An efficient method was established for the separation and preparation of chlorogenic acid,pedaliin-6″-acetate and rosmarinic acid from Dracocephalum tanguticum Maxim.. The chlorogenic acid,pedaliin-6″-acetate and rosmarinic acid was enriched by semi-preparative high performance liquid chromatography（SPHPLC） from ethyl acetate extract,and then separated and purification by high-speed counter-current chromatography（HSCCC）and preparative high performance liquid chromatography（Pre-HPLC）. A total of 54 mg of chlorogenic acid,150 mg of pedaliin-6″-acetate and 200 mg of rosmarnic acid were obtained from ethyl acetate extract with purities of 96.9%,97.9% and 95.1% by HPLC analysis,respectively. Their structures were identified by nuclear magnetic resonance spectrum. The result show that this method is suitable for the isolation and purification of chlorogenic acid,pedaliin-6″-acetate and rosmarnic acid from D. tanguticum. It also avoids the disadvantages of traditional separation methods such as many operations,large reagent consumption and non-recyclability,provides a reference basis for the study of separating of active components and preparation reference substance from D. tanguticum.