Abstract: 14-3-3 protein,also called general regulatory factors（GRF）,is a type of serine and threoninephosphorylation binding protein,which participates in the regulation of series of physiological processes,suchas intracellular basis,signal transduction,plant growth and development,environmental stress response,byinteracting with other transcription factors or signaling proteins. In this study,the ThGRF2 gene that wasdifferentially expressed under drought stress and cloned from the drought transcriptome of Tamarix hispida. TheCDS fragment of ThGRF2 gene was 786 bp,encoded 261 amino acids. The relative molecular weight was 29.40k Da,and the theoretical isoelectric point was 4.76. The overexpression vector p ROKII-ThGRF2 wasconstructed,and transformed into T. hispida. The physiological index results showed that ThGRF2 overexpressionincreased the chlorophyll content,SOD and POD activities,and reduced malondialdehyde content（MDA）,electrical conductivity（EL）and water loss rate under osmotic stress,indicated that ThGRF2 gene played animportant role in the response to osmotic stress of T.hispida. It laid a foundation for further exploration of theabiotic stress tolerance function of ThGRF2 gene.