Abstract: In order to explore the flowering strategies of early spring flowering plants at low temperatures,five early spring flowering plants and five non-early spring flowering plants were collected,and the endogenous hormones and secondary metabolites between early spring flowering plants and non-early spring flowering plants were analyzed by high performance liquid chromatography-mass spectrometry and metabolomics.The results showed that the content of auxin（IAA）and zeatin（ZT）in early spring flowering plants were significantly lower than that in non-early spring flowering plants,while gibberellin（GA₃） and abscisic acid（ABA） were significantly higher than that in non-early spring flowering plants,indicated that GA₃ and ABA might play a regulatory role in promoting the flowering process of early spring plants.In the secondary metabolites,L-phenylalanine,the precursor of phenolic metabolites,was usually higher in non-early spring flowering plants than in early spring flowering plants.In addition,there were C6C1 phenolic compounds,including vanillic acid,syringic acid and protocatechuic acid;C6C3 phenolic compounds,including ferulic acid,chlorogenic acid and caffeic acid;Flavonoids,including apigenin,genistein,hesperidin,chrysin and galangin,were generally higher in non-early spring flowering plants than in early spring flowering plants.The changes of these secondary metabolites might be due to a large amount of material loss caused by the flowering process of flowering plants in early spring,which reduced the level of secondary metabolism.The results showed that endogenous plant hormones and secondary metabolites regulated the physiological process of flowering plants in early spring,and laid a foundation for further understanding the flowering process of plants under low temperatures.