Abstract: To screen out the tomato（Solanum） germplasm with waterlogging tolerance, 50 tomato germplasm were selected as the research object, and the survival rate and physiological indexes were compared after semisubmerged and submerged treatments respectively. The results showed that 12 waterlogging tolerant tomato germplasm with 100% survival rate after seven days submergence treatment were selected, and 12 waterlogging-tolerant germplasm were observed and distributed in S. lycopersicum,S. lycopersicum var. cerasiforme and S. pimpinellifolium respectively. Further analysis on waterlogging-tolerant tomato TK017 and waterloggingsensitive tomato TK039 revealed that tomato TK017 could control the content of reactive oxygen species more effectively under submergence condition, and improved the waterlogging tolerance of tomato.