Abstract: In order to further understand the pathway of anthocyanin synthesis,the four tissues of C.gracile,namely,root,stem,leaf and flower,were sequenced using BGISEQ-500 platform.128 856 unigenes were assembled and 40 unigenes encoding six key enzymes involved in the anthocyanin biosynthesis were identified.Homologous alignment and structure simulation of bifunctional dihydroflavonol 4-reductase（DFRs） were carried out,which showed that they have well-conserved sequences,spatial structures and NAD+binding sites.The DFR structure model showed a tetrameric structure with Rossmann-like α-helixes/β-sheets/α-helixes sandwich fold.