Abstract: To clarify the potential function of Glutathione-S-transferase gene（GST）family in sugar beet（Beta vulgaris）growth and development and abiotic stress,the sugar beet Bv GSTs was identified,and the physical and chemical properties,evolutionary relationship,homeopathic elements,chromosome location and transcriptional expression characteristics under cadmium stress were comprehensively analyzed,respectively.The results showed that there were 52 members of Bv GSTs in sugar beet genome,which were distributed in seven subfamilies;The molecular weight of the members ranged from 17-28 k Da,and the theoretical isoelectric point（p I）was between 5.16-6.77,and most of the members were located in cytoplasm.There were nine motifs found in Bv GSTs,among which motif eight was unique to Phi subfamily.Among all the members,30Bv GSTs were distributed in eight chromosomes respectively,with four tandem repeats.According to cis-acting element analysis,Bv GSTs might be involved in a variety of biotic and abiotic stress responses.Transcriptome analysis showed that all of 52 Bv GSTs were related to response of sugar beet to cadmium stress.The expression of most members of Tau subfamily under ground parts was positively regulated by cadmium stress;Phi subfamily above ground parts was significantly induced by cadmium stress,and the expression of Tau family was inhibited.The result of q RT-PCR showed that the transcripts of the four differentially expressed Bv GSTs were regulated by cadmium stress,which was consistent with the results of transcriptome sequencing.These results would play a foundamental role in further study on the biological function of sugar beet glutathione S-transferase under cadmium stress.