Abstract: To explore the effect of exogenous NO donor（sodium nitroprusside,SNP）on soil nutrient contentand Catharanthus roseus seedlings growth under light-shading stress,four treatments（normal culture,externalapplication of SNP,light-shading treatment and external application of SNP+ shading treatment） weredesigned,and the change characteristics of C,N,P nutrient contents and physicochemical index from pottedsoil,and the seedling shoot growth index（plant height,leaf length,leaf width,leaf area,fresh mass and drymass）were analyzed respectively. The results showed that soil water content,soil p H and soil organic carboncontent increased significantly（P＜0.05）under the combination of shading stress and exogenous NO,while thecontents of total nitrogen,alkali-hydrolyzable nitrogen,total phosphorus and available phosphorus in soilincreased but not significantly,and the soil C/N and C/P values reached significant level,and the C/N value increased significantly（P＜0.05）;Correlation analysis showed that there was a positive correlation between soilp H value and organic carbon content（P＜0.05）,the change of soil organic carbon content was positively correlatedwith plant height and leaf fresh mass（P＜0.05）. It indicated that external application of SNP could promote theincrease of soil p H under light-shading stress,change soil organic carbon content,increase C/N value,improvesoil fertility,and promote leaf growth and development,then increase the biomass of C. roseus,and provide basisfor scientific balanced fertilization and alkaloid content accumulation of C. roseus.