Abstract: A total of 18 new StCRKs（cysteine-rich receptor-like kinases,CRKs）family genes were identified in potato genome using online tools such as Pfam. These StCRKs localize on chromosome 1,2,11 and 12,and all contain conserved CRK domains. In the promoter regions of these StCRKs genes,there are five plant hormones responsive elements,the circadian control element,biotic and abiotic responsive elements,and seedspecific regulation element. The tissue-specific expression pattern were measured using qRT-PCR method in root,stem,leave,and flower tissues in potato during flowering time. The results showed different StCRKs expressed in different tissues. After salicylic acid analogue BTH and 4 ℃ treatment,it was found that 10StCRKs genes could be induced by BTH and 13 StCRKs genes were able to respond to the low temperature signal. The results provide clues for further elucidation functions of StCRKs on biotic and abiotic stresses.