Abstract: To explore the effects of different nutrient additions on the growth and nutrient content of Populus euramericana"N3016"× P. ussuriensis,Populus simonii × Populus nigra and Populus ussuriensis,and to revealthe nutrient demand and utilization rules of poplar,and to provide a reference for nursery cultivation of poplarseedlings,P. euramericana"N3016"× P. ussuriensis,Populus simonii × Populus nigra and Populus ussuriensis were used as materials,3 factors（N:nitrogen,P:phosphorus,K:potassium）and 3 levels（N:1.5,3,6 g;P:0.75,1.5,3 g;K:0.5,1,2 g,for per plant respectively.）L9（34）orthogonal experimental design were uesd,plants with no fertilization as control,and the effects of different nutrient supply levels on the growth dynamicsand nutrient distribution of different poplars were studied respectively. The results showed that:（1） Differentnutrient supply had significant effects on the growth of seedling height and ground diameter of P. euramericana"N3016"× P. ussuriensis,Populus simonii × Populus nigra and Populus ussuriensis from the middle of July. The height and base diameter growth index of three tree species was the highest at NPK 1 level respectively. Thebiomass of P. euramericana"N3016"× P. ussuriensis was the highest when N,P and K was selected at 3,1 and2 level respectively. The biomass of Populus simonii × Populus nigra was the highest when N,P and K level was2,1,1,respectively. The biomass of Populus ussuriensis was the highest when N,P and K level was 1,respectively. The effects of N,P and K on the growth indexes of P. euramericana"N3016"× P. ussuriensis andPopulus simonii × Populus nigra was N＞P and K,while the effects of N,P and K on the growth indexes ofPopulus ussuriensis was K＞N and P.（2）The accumulation of nitrogen,phosphorus and potassium in roots,stemsand leaves of P. euramericana"N3016"× P. ussuriensis,Populus simonii × Populus nigra and Populus ussuriensis was N＞P,K. The accumulation of nitrogen in each organ of the 3 varieties was greater than that ofphosphorus and potassium. Among the 3 poplar varieties,the accumulation of 3 kinds of nutrients in each organwas significantly different. The distribution ratio of total nitrogen accumulation in 3 varieties was stem＞leaf＞root.The distribution ratio of phosphorus accumulation of P. euramericana"N3016"× P. ussuriensis and Populus simonii × Populus nigra was stem＞leaf＞root. The order of Populus ussuriensis was leaf＞stem＞root. Thedistribution of potassium accumulation of P. euramericana"N3016"× P. ussuriensis and Populus ussuriensis wasleaf＞stem＞root. The distribution of potassium accumulation of Populus simonii × Populus nigra was higher thanthat of stem＞leaf＞root.（3）Through the comprehensive analysis of each index,it was concluded that the optimalfertilization scheme of Populus ussuriensis,P. euramericana"N3016"× P. ussuriensis and Populus simonii ×Populus nigra was N1 P1 K1,that was,N 1.5 g,P 0.75 g,K 0.5 g,for per plant respectively.