Abstract: To assess the size and complexity of the genome of the endangered plant Tetraena mongolica, and reveal the hyper-arid mechanism of T. mongolica and further explore its characteristic genetic resources, and to better decipher the whole genome information of T. mongolica, the second-generation high-throughput sequencing technology of genome survey analysis was used as the method, and bioinformatics method was used to estimate the genome information such as heterozygosity, repeat sequence and GC content of T. mongolica respectively. The results showed that the genome size of T. mongolica was 1 079.25 Mb, the corrected genome size was 1 065.84 Mb, and the heterozygosity rate was 0.76%, the percentage of repetitive sequences was 75.25%, and the GC content was 33.57% respectively. After the initial assembly of T. mongolica genome 3 502 126contigs were obtained, totaling 682 Mb, with an N50 of 187 bp. It was hypothesized that T. mongolica genome belonged to a homo-tetraploid complex genome, which was difficult to assemble by whole-genome sequencing.Due to the high heterozygosity of T. mongolica, the third-generation high-throughput sequencing technology（single-molecule sequencing） combined with chromatin region capture technology could be used subsequently, which was expected to finally obtain a high-quality whole-genome map of T. mongolica.