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中国‘苦水’玫瑰杂交育性的初步研究

Preliminary Study on Cross Fertility in ’Kushui’ Rose

  • 摘要: 油用玫瑰主栽品种‘苦水’玫瑰的栽培园区总见千花无果现象,为此,本研究对‘苦水’玫瑰的杂交育性进行了遗传学研究。授粉试验发现,自然授粉和人工辅助授粉时‘苦水’玫瑰自交不结实;异交时‘,苦水’玫瑰作为母本与‘丰花’玫瑰、‘四季’玫瑰、‘艳霞’玫瑰、重瓣黄刺玫之间具有亲和性,而作为父本与杂交母本都无法成功授粉结实。在雄蕊花瓣化、P/O值、花粉异型性和花粉萌发率等生殖特征中发现‘苦水’玫瑰的花粉畸形现象严重,萌发率近似为零。进而,显微观察小孢子母细胞减数分裂各时相发现‘苦水’玫瑰中存在染色体分配行为不协调,胞质分裂不均一,四分体中有三分体等异常现象。由此可见‘苦水’玫瑰的异常减数分裂行为致花粉败育妨碍其做父本时的受精结实,故雄性不育而非蔷薇科常见的自交不亲和性,是‘苦水’玫瑰自交不结实的主要原因,也表明作为母本通过杂交育种方法改良‘苦水’玫瑰的种质是可行的。

     

    Abstract: In order to explore the reason why there is no fruit in autumn despite of thousands of flowers of’Kushui’rose(Rosa sertate × R.rugosa Yu.et.Ku.)blossoming in garden,we conducted a genetic research onits cross fertility in this study.The results showed that the selfing of’Kushui’rose did not bear fruit in nature orby artificial pollination.Meanwhile,there were no fruit obtained once pollen of’Kushui’rose being pollinatedon the stigmas of the other varieties in Rosa.In contrast to this,’Kushui’rose could produce amount of seedswhen pollinated with pollen from the same other materials.All evidences supported that the pollen of’Kushui’rose rose was sterile.Further,several reproductive features,such as stamen petallization,P/O value,pollenheteromorphorsis,and pollen germination rate were studied,with the results showing the pollen of’Kushui’rose deformed so seriously that the germination rate was nearly zero.Furthermore,microscopic observation ofpollen mother cells at different phases of meiosis revealed there were so much abnormal behaviors ofchromosome allocation as well as unequal cytokinesis that plenty of triads were yielded that coexisting withdeformed tetrad in pollen sac.In conclusion,the study shows that the abnormal division of pollen mother cells of’Kushui’rose is responsible for the pollen sterility and no fruit setting when used as paternal parent inhybridization.And that pollen abortion causing male sterile,rather than self-incompatibility commonlydistributing in Rosaceae,is responsible for the intraspecific cross infertility of’Kushui’rose,further implyingit is feasible to improve its germplasm by acting as a maternal in cross-breeding.

     

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