Abstract: As a kind of medicinal herb,Sanicula rubriflora F.Schmidt is easily confused with other medicinal plants of the same genus after drying and interspecific relationship is controversial.The structure and characteristics of the chloroplast genome of S.rubriflora were reported for the first time,the sequencing data was spliced and annotated using bioinformatics methods,and the chloroplast genome structure and phylogeny were further analyzed.The length of the chloroplast genome sequence of S.rubriflora was 155 721 bp,including a large single copy region（LSC）of 85 981 bp and a small single copy region（SSC）of 17 060 bp,and were separated by inverted repeat sequence（IRs）of 26 340 bp.The GC content of the chloroplast genome of S.rubriflora was 38.20% and contained 129 genes,84 protein-coding genes,37 tRNA genes and 8 rRNA genes respectively.The chloroplast genome structure of S.rubriflora was highly conservative.The coding genes contained a total of 51 907 codons,with 5 095 leucine and 689 tryptophan.Simple repeated sequence analysis revealed a total of 32 sites,and most of them were single base repeat A/T type.The clustering results of the chloroplast genome supported that the subfamily Hydrocotyloideae was a relatively primitive group in the Umbelliferae family.The subfamily Saniculoideae and Apioideae were sister groups,which were the more close relationship of the Umbelliferae.Sanicula was a relatively natural group.S.rubriflora and S.flavovirens were related sister species,but their morphology and geographical distribution were quite different.The results provid a basis for the interspecies identification of the genus Sanicula and lay the foundation for further study of its phylogenetic relationship and interspecies evolution.