Callus Induction and Somatic Embryogenesis and Physiological State Analysis from Mature Zygotic Embryo Explant of Syringa reticulata var. mandshurica

In order to optimize somatic embryo induction system of Syringa reticulata var. mandshurica,callus induction and somatic embryogenesis from mature zygotic embryo were conducted by adjusting the type and concentration of plant growth regulators. The morphological observation and physiological analysis of the explants in the culture process were carried out. The results showed that:（1）Somatic embryo regenerate from mature zygotic embryo explant 30 d cultured later and cotyledonous somatic embryo can be obtained cultured 60 d later;（2）BA played a leading role in callus induction,the callus induction rate was reached 100% in the combinations of 0.5 mg·mL^-1 BA with 5-6 mg·mL^-1 NAA,and the somatic embryo induction rate reached 8% in the combination of 0.5 mg·mL^-1 BA and 5 mg·mL^-1 NAA;（3） Polyphenol content increased sharply at the primary stage of callus formation and kept a relatively high level in the process of culture. The PAL and POD activity increased and the MDA and SOD activity decreased in cotyledonous stage of somatic embryo.