Identification of Key Enzyme Genes Involved in Anthocyanin Synthesis pathway in Clinopodium gracile by Transcriptome Analysis

In order to further understand the pathway of anthocyanin synthesis,the four tissues of C.gracile,namely,root,stem,leaf and flower,were sequenced using BGISEQ-500 platform.128 856 unigenes were assembled and 40 unigenes encoding six key enzymes involved in the anthocyanin biosynthesis were identified.Homologous alignment and structure simulation of bifunctional dihydroflavonol 4-reductase（DFRs） were carried out,which showed that they have well-conserved sequences,spatial structures and NAD+binding sites.The DFR structure model showed a tetrameric structure with Rossmann-like α-helixes/β-sheets/α-helixes sandwich fold.