Simultaneous Determination of Baschnaloside and Rossicaside B in Boschniakia rossica by HPLC

In order to establish an HPLC method for the simultaneous determination of baschnaloside androssicaside B in Boschniakia rossica dry whole,a chromatographic column:Klimail 100-5 C18 column（250 mm×4.6 mm,5 μm）;mobile phase:acetonitrile as mobile phase A,0.5% formic acid aqueous solution as mobilephase B,gradient elution conditions:0-12 min,15% A;12-30 min,15%-20% A;30-40 min,20%-25%A;40-45 min,25%-30% A;45-60 min,30%-100% A;flow rate:1.0 m L·min^-1;detection wavelength:260 nm. Column temperature:30℃. Injection volume:20 μL. The linear range of naringin of baschnaloside was4.688-150 μg·g^-1（R2=0.999 5）;the linear range of rossicaside B was 3.438-110 μg·g^-1（R2=0.999 1）;theaverage recoveries were 97.86% and 96.55%,and RSDs were 1.01 and 1.23（n=9）,respectively. A highperformance liquid chromatography method was developed for the simultaneous determination of twocomponents,baschnaloside and rossicaside B in B.rossica. The methodological verification results show that themethod is simple,fast,and reproducible,and can be used to determine the contents of baschnaloside androssicaside B in B.rossica.