Optimization of Liquid Fermentation Culture Medium for Inducing Enrichment of Extracellular Ganoderic Acid A

The formula of liquid culture medium, which induced the enrichment of Ganoderma triterpenoid ganoderic acid A, was optimized using the response surface method. The optimal formula was as followed: glucose35 g, histidine 0.7 g, complex B vitamins 40 mg （VB₁6 mg, VB₂3 mg, VB₆0.4 mg, niacinamide 20 mg, calcium pantothenate 2 mg）, taurine 0.1 g, and distilled water 1 000 mL. Adjusting pH to 6.4 with 5% NaOH and 5% HCl.At 26 ℃ and 160 r·min^-1, the mycelium biomass of Ganoderma lucidum on the medium was 11.45 g·L^-1, and the yield of ganoderic acid A was 137.98 mg·L^-1.