Morphological observation of Ascaridia galli eggs and Heterakis gallinarum eggs
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摘要: 为了了解鸡蛔虫卵与异刺线虫卵形态结构的区别,试验采用全身性寄生虫学剖检法,从锦州地区农村散养的家鸡小肠和盲肠中采集鸡蛔虫和异刺线虫的成虫,各取5条雌虫,剪碎、挤压后取其虫卵。利用普通光学显微镜和扫描电镜观察的方法,对两种虫卵的大小和形态进行了对比。结果表明:鸡蛔虫卵呈短椭圆形、浅灰色,形似橄榄状,长径为(77.61±3.91)μm,宽径为(44.32±3.08)μm,长径与宽径比为1.76±0.15,卵壳表面不光滑,有少量脊状突起和纵沟,有丝络样小孔;异刺线虫卵呈长椭圆形、淡灰褐色,形似枕头,长径为(66.26±3.85)μm,宽径为(37.20±2.98)μm,长径与宽径比为1.79±0.17,卵壳表面有数条纵脊,呈均匀的布纹样。说明两种虫卵在两种光学显微镜下形态很相似,扫描电镜可看到虫卵表面的细微结构,值得进一步研究。Abstract: In order to understand the differences between the morphological structure of Ascaridia galli eggs and Heterakis gallinarum eggs. the experiment was conducted by systemic parasitological dissection method, and Ascaridia galli eggs and Heterakis gallinarum adults were collected from the small intestine and cecum of free-ranging domestic chickens in the Jinzhou region.Five female worms were collected from the adult worms of Ascaridia galli and Heterakis gallinarum; the eggs were obtained after cutting and squeezing. The size and morphology of the two kinds of eggs were compared by using ordinary optical microscope and scanning electron microscope. The results showed that the eggs of Ascaridia galli were short oval, light gray, olive-like, with a length diameter of(77.61±3.91) μm, a width diameter of(44.32±3.08) μm, and a length-to-width-diameter ratio of 1.76±0.15; the surface of the egg shell was not smooth, with a few ridges and longitudinal grooves, and there were filamentous pores. The eggs of Heterakis gallinarum were oblong, light gray-brown, pillow-like, with a length diameter of(66.26±3.85) μm, a width diameter of(37.20±2.98) μm, and a length-to-width-diameter ratio of 1.79±0.17; there were several longitudinal ridges on the surface of the eggshell, and uniform cloth pattern could be seen. The results suggested that the morphology of the two species of eggs was very similar under light microscopy, and scanning electron microscope could see the fine structure of the egg surface, which was worthy of further study.
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Keywords:
- Ascaridia galli /
- Heterakis gallinarum /
- eggs /
- shell /
- morphology /
- size
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