植物材料减数分裂染色体标本的快速制作

丁海燕

doi:10.7525/j.issn.1673-5102.2017.05.021

植物材料减数分裂染色体标本的快速制作

丁海燕

大庆师范学院, 大庆 163712

基金项目:

大庆师范学院科学研究基金项目（12ZR04）；大庆师范学院教改项目（JY1511）

详细信息

作者简介:
丁海燕(1973-),女,博士,副教授,主要从事遗传学教学与研究。

中图分类号: Q243
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出版历程
- 收稿日期: 2017-01-03
- 刊出日期: 2017-09-19

Fast Manufacture of Meiotic Chromosome Specimens of the Plant Material

DING Hai-Yan

Daqing Normal University, Daqing 163712

Funds:

Natural Science Foundation of Daqing Normal University(12ZR04)

摘要

摘要: 探索一种简便快速的植物材料减数分裂染色体标本制作技术，获得良好的减数分裂染色体标本以供原位杂交研究、遗传学实验教学等方面研究使用。以植物材料小麦、黑麦花药为材料，卡宝品红染色，冰冻揭片法制得减数分裂染色体标本，结果表明，这些用快速简便的方法获得的标本效果良好，染色体图像清晰，可较好的用于本科生实验教学、染色体分带、原位杂交等方面的研究。
- 植物材料 /
- 减数分裂 /
- 染色体标本
Abstract: In order to establish a set of simple techniques for meiosis chromosome specimen preparation of pollen mother cell for in situ hybridization study, genetics experimental teaching, etc. Wheat and rye anther was used as experimental materials, glass slides were prepared by the improved method, and the meiosis of pollen mother cell were observed under microscope and recorded by photograph. These permanent specimen were good, showing the dynamic continuous phases of meiosis. They could be better used in the experiment teaching and in situ hybridization study.
- plant material /
- meiosis /
- chromosome specimen

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参考文献(20)

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