Application Progress of High Speed Countercurrent Chromatography in the Study of Natural Products
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摘要: 高速逆流色谱是液-液分配色谱,与传统固-液分配色谱相比,其具有固定相成本低、易回收、分离过程灵活、高效等优点,近年来被广泛应用于天然产物研究中。本文总结归纳了高速逆流色谱的两相溶剂体系、分离洗脱方式的选择和应用方法,分析阐述了高速逆流色谱技术在天然产物研究中的应用实例,为天然产物功效成分的进一步深入研究提供可靠的技术参考。Abstract: High speed countercurrent chromatography is liquid-liquid partition chromatography. Compared with traditional solid-liquid partition chromatography, it has been widely used in recent years because of its advantages of low cost, easy recovery, flexible and efficient separation process. This paper summarized the selection skills of two-phase solvent system and the application methods of separation and elution by analyzing and elaborating the application examples of high-speed countercurrent chromatography technology in the study of natural products.The flexible and efficient characteristics of high-speed countercurrent chromatography separation technology was fully demonstrated in this review. Reliable technical references for the further study of functional components of natural products were provided.
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